Goat Anti-Androgen Receptor Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

RP11-383C12.1|dihydrotestosterone receptor|androgen receptor|HYSP1|HUMARA|SMAX1|NR3C4|SBMA|DHTR|TFM|AIS|KD|AR|dihydrotestosterone receptor|androgen receptor

Immunofluorescence: Strong expression of the protein seen in the mitochondria/cytoplasm of MCF7 and U2OS cells. Recommended concentration: 10µg/ml.

Chromatin Immunoprecipitation: A six-fold increased presence of the FKBP5 locus observed when comparing the precipitations using EB06441 with non-specific goat IgG to bring down Androgen Receptor in a chromatin lysate from DHT-stimulated HEC50 cells.

NP_000035.2

EBP06441

Peptide with sequence EVQLGLGRVYPRPPSC, from the N Terminus of the protein sequence according to NP_000035.2.

This antibody is expected to recognize isoform 1 (NP_000035.2) only.

EVQLGLGRVYPRPPSC

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Dog, Pig, Cow

Human, Mouse

367

11835

24208

In paraffin embedded Human Prostate shows nuclear staining in the secretory cells of the gland. Recommended concentration: 2-3µg/ml. Cryosections of Mouse Hypothalamus and Amygdala. Recommended dilution range: 1:20,000.

Antibody detection limit dilution 1:64000.

Approx 100kDa band observed in Human Cerebellum lysates (calculated MW of 99.2kDa according to NP_000035.2). Recommended concentration:0.1-0.5µg/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Pancreas lysate. Preliminary testing was unsuccessful on Mouse and Rat for this particular batch.

Pep-ELISA, WB, IHC, IF, ChIP