Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names OTTHUMP00000016257|OTTHUMP00000016256|peroxisome proliferator-activated receptor delta|nuclear hormone receptor 1|peroxisome proliferative activated receptor, delta|PPAR-beta|MGC3931|PPARB|NUCII|NR1C2|NUCI|NUC1|FAAR|PPARD | |
Accession ID NP_006229.1 | |
Blocking Peptide EBP06474 | |
Immunogen Peptide with sequence CHPLLQEIYKDMY, from the C Terminus of the protein sequence according to NP_006229.1. | |
Product Comments This antibody is expected to recognise isoform 1 (NP_006229) of PPAR delta, but not isoform 2, a shorter protein (NP_803184) with a different C-terminus. | |
Peptide Sequence CHPLLQEIYKDMY | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Pig, Cow | |
Reactive Species Human | |
Human Gene ID 5467 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:32000. | |
Western Blot Approx 55kDa band observed in T cell line MOLT4 lysates (calculated MW of 49.9kDa according to NP_006229.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour. | |
Application Type Pep-ELISA, WB |
Goat Anti-PPAR delta (Isoform 1) Antibody
$423.00
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Selected References [{"pmid": 20215574, "intro": "This antibody (previous batch) has been successfully used in Western blot on Rat:", "title": "Iron restriction improves type 2 diabetes mellitus in Otsuka Long-Evans Tokushima fatty rats.", "author": "Minamiyama Y, Takemura S, Kodai S, Shinkawa H, Tsukioka T, Ichikawa H, Naito Y, Yoshikawa T, Okada S", "journal": "Am J Physiol Endocrinol Metab. 2010 Jun; 298(6):E1140-9."}] |