Goat Anti-ERK2 / MAPK1 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB06592
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
extracellular signal-regulated kinase 2|mitogen-activated protein kinase 2|protein tyrosine kinase ERK2|mitogen-activated protein kinase 1|p41mapk|P42MAPK|PRKM2|PRKM1|MAPK2|ERT1|p41|p40|p38|ERK|ERK2|MAPK1
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 cells. Recommended concentration: 10µg/ml.
Accession ID
NP_002736.3; NP_620407.1
Blocking Peptide
EBP06592
Immunogen
Peptide with sequence CAAGPEMVRGQVF, from the internal region of the protein sequence according to NP_002736.3; NP_620407.1.
Product Comments
This antibody is expected to recognise an epitope corresponding to aa 9-19 of both reported protein isoforms of human ERK2 / MAPK1 protein.
Peptide Sequence
CAAGPEMVRGQVF
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Cow
Reactive Species
Human
Human Gene ID
5594
Mouse Gene ID
26413
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:8000.
Western Blot
Approx 38kDa band observed in HepG2 lysates (calculated MW of 41.4kDa according to NP_002736 and NP_620407). Recommended concentration: 0.5-2µg/ml. Primary incubation was 1 hour.
Application Type
Pep-ELISA, WB, IF

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