Goat Anti-IRAK4 (N Terminus) Antibody

$464.00

Category: Primary Antibodies
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SKU: EB06619
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
IPD1|interleukin-1 receptor associated kinase 4 mutant form 1|interleukin-1 receptor associated kinase 4|IRAK-4 mutated form 1|interleukin-1 receptor-associated kinase 4|NY-REN-64|REN64|IRAK4
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm and microtubules of HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Accession ID
NP_057207.2; NP_001107654.1
Blocking Peptide
EBP06619
Immunogen
Peptide with sequence NKPITPSTYVRC, from the N Terminus of the protein sequence according to NP_057207.2; NP_001107654.1.
Peptide Sequence
NKPITPSTYVRC
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse
Reactive Species
Human
Human Gene ID
51135
Mouse Gene ID
266632
Product Grade
IHC Results
Paraffin embedded Human Heart and Human Liver. Recommended concentration: 3.75µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 50kDa band observed in lysates of cell line HeLa and approx 55kDa in lysates of cell line Jurkat (calculated MW of 51.5kDa according to NP_057207.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.5-2µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IHC, IF, FC

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