Goat Anti-DYX1C1 (Isoform a) Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

RD|MGC70618|dyslexia susceptibility 1 candidate 1|FLJ37882|DYXC1|EKN1|DYX1|DYX1C1

NP_570722.2

EBP06656

Peptide with sequence C-KIRNVIQGTELKS, from the C Terminus of the protein sequence according to NP_570722.2.

This antibody is expected to recognise only one of the three reported isoforms (NP_570722.2, isoform a).

C-KIRNVIQGTELKS

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat

Human

161582

67685

In paraffin embedded Human Cerebral Cortex shows cytoplasm staining in some of the neuronal cells. Recommended concentration, 5-10µg/ml.

Antibody detection limit dilution 1:16000.

Lysates of COS1 transfected with full length recombinant Human DYX1C1 gave bands at approx 48kDa after 0.1µg/ml antibody staining. In addition, a minor band of 24kDa is detected consistent with observations with N-terminal specific antibodies (unpublished data, LoTurco, Connecticut, USA). The 75kDa band is visible in the non-transfected COS1 also (first lane) and is therefore non-specific. We call for caution when this product is used in other assays than Western blot. Data kindly provided by Wang and LoTurco, University of Connecticut, USA.

Pep-ELISA, WB, IHC