Goat Anti-ACHE Antibody

$464.00

Category: Primary Antibodies
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SKU: EB06659
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
acetylcholinesterase; apoptosis-related acetylcholinesterase|OTTHUMP00000211356|OTTHUMP00000211349|OTTHUMP00000211347|N-ACHE|ARACHE|acetylcholinesterase (YT blood group)|YT|ACHE
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm, plasma membrane and nucleus of U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Accession ID
NP_000656.1; NP_001269378.1
Blocking Peptide
EBP06659
Immunogen
Peptide with sequence QFDHYSKQDRCSDL, from the C Terminus of the protein sequence according to NP_000656.1; NP_001269378.1.
Product Comments
This antibody is expected to recognise isoform NP_000656.1 only (the ubiquitously expressed, hydrophillic form).
Peptide Sequence
QFDHYSKQDRCSDL
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Rat, Mouse
Reactive Species
Human
Human Gene ID
43
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 70kDa band observed in HepG2 and Jurkat cell lysates (calculated MW of 67.8kDa according to NP_000656.1). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, FC

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