Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names nanos homolog 1|NOS1|nanos homolog 1 (Drosophila)|NANOS1 | |
Accession ID NP_955631.1 | |
Blocking Peptide EBP06680 | |
Immunogen Peptide with sequence C-SARDGPPGKKLR, from the C Terminus of the protein sequence according to NP_955631.1. | |
Peptide Sequence C-SARDGPPGKKLR | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human | |
Reactive Species Human | |
Human Gene ID 340719 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:64000. | |
Western Blot Approx 35-37kDa band observed in Human Testis lysates (calculated MW of 30.2kDa according to NP_955631.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Clarke et al, Hum Mol Genet. 2004 Apr 1;13(7):727-39. Epub 2004 Feb 12, PMID: 14962983). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour. This antibody has been successfully used in WB on Human: Sajek et al (2018) PMID: 30269240. | |
Application Type Pep-ELISA, WB |
Goat Anti-NANOS1 Antibody
$423.00
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Selected References [{"pmid": 30269240, "intro": "This antibody has been successfully used in Western blot on Human:", "title": "PUM1 and PUM2 exhibit different modes of regulation for SIAH1 that involve cooperativity with NANOS paralogues", "author": "Sajek M, Janecki DM, Smialek MJ, Ginter-Matuszewska B, Spik A, Oczkowski S, Ilaslan E, Kusz-Zamelczyk K, Kotecki M, Blazewicz J, Jaruzelska J.", "journal": "Cell Mol Life Sci. 2018 Sep 29. "}] |