Goat Anti-BID Antibody

$423.00

Category: Primary Antibodies
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SKU: EB06713
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
desmocollin type 4|OTTHUMP00000196197|BID isoform Si6|BID isoform L(2)|BID isoform ES(1b)|FP497|apoptic death agonist|Human BID coding sequence|BH3-interacting domain death agonist|MGC42355|MGC15319|HGNC:1050|BH3 interacting domain death agonist|BID
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of MCF7 cells. Recommended concentration: 10µg/ml.
Accession ID
NP_932070.1; NP_001187.1; NP_932071.1
Blocking Peptide
EBP06713
Immunogen
Peptide with sequence C-TYVRSLARNGMD, from the C Terminus of the protein sequence according to NP_932070.1; NP_001187.1; NP_932071.1.
Product Comments
This antibody is expected to recognise all three reported isoforms (as represented by NP_932070.1; NP_001187.1; NP_932071.1)
Peptide Sequence
C-TYVRSLARNGMD
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
637
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:8000.
Western Blot
Approx 26+24kDa bands observed in A431 cell lysates (calculated MW of 26.8kDa according to NP_932070.1 and 22.0kDa according to NP_001187.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF

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