Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names SERPINE1|serpin E1|plasminogen activator inhibitor-1|plasminogen activator inhibitor, type I|endothelial plasminogen activator inhibitor|plasminogen activator inhibitor-1|serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1|plasminogen activator inhibitor, type I|serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1|PLANH1|PAI-1|PAI|PAI1|SERPINE1 | |
Accession ID NP_000593.1 | |
Blocking Peptide EBP06720 | |
Immunogen Peptide with sequence C-GFKIDDKGMAPALRH, from the internal region of the protein sequence according to NP_000593.1. | |
Peptide Sequence C-GFKIDDKGMAPALRH | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Dog | |
Reactive Species Human | |
Human Gene ID 5054 | |
Product Grade  | |
IHC Results In paraffin embedded Human Placenta shows strong staining of cytotrophoblasts. Recommended concentration: 4-6µg/ml.
A customer successfully used this product on Human Breast cancer. | |
ELISA Detection Limit Antibody detection limit dilution 1:64000. | |
Western Blot Approx 48kDa band observed in Human Liver and HeLa lysates, while approx. 45kDa observed in lyates of cell lines A549 and HepG2 (calculated MW of 45.1kDa according to NP_000593.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour. | |
Application Type Pep-ELISA, WB, IHC |
Goat Anti-PAI1 / SERPINE1 Antibody
$423.00
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Selected References [{"pmid": 23769831, "intro": "This antibody has been successfully used in the following paper:", "title": "Bacterial expression, purification and angiogenesis-promoting activity of human thymosin ?4.", "author": "Kozaczuk A, Selmi A, Bednarek R.", "journal": "Protein Expr Purif. 2013 Aug;90(2):142-52."}] |