Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size 100 µg
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names arrestin beta-2\|arrestin 3\|BARR2\|beta-arrestin 2\|arrestin beta 2\|DKFZp686L0365\|ARR2\|ARB2\|HGNC:712\|ARRB2\|arrestin, beta 2
Usage Summary Immunofluorescence: Strong expression of the protein seen in HeLa cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.
Accession ID NP_004304.1; NP_945355.1; NP_001244257.1; NP_001244259.1; NP_001244260.1; NP_001316993.1
Blocking Peptide EBP06745
Immunogen Peptide with sequence C-HDHIPLPRPQS, from the internal region of the protein sequence according to NP_004304.1; NP_945355.1; NP_001244257.1; NP_001244259.1; NP_001244260.1; NP_001316993.1.
Product Comments This antibody is expected to recognise reported isoforms 1, 2, 3, 5, 6 and 7. No crossreactivity is expected with Arrestin beta 1.
Peptide Sequence C-HDHIPLPRPQS
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions Refrigerated
Predicted Species Human, Mouse, Rat
Reactive Species Human
Human Gene ID 409
Mouse Gene ID 216869
Rat Gene ID 25388
Product Grade
ELISA Detection Limit Antibody detection limit dilution 1:64000.
Western Blot Approx 45kDa band observed in lysates of cell lines A549 and U251 and in preliminary testing of KNRK cell lysate (calculated MW of 46.1kDa according to NP_004304.1 and 44.4kDa according to NP_945355.1) Recommended concentration: 0.5-1µg/ml. Primary incubation 1 hour at room temperature.
Application Type Pep-ELISA, WB, IF, FC

Selected References

[{"pmid": 20227500, "intro": "This antibody (previous batch) has been successfully used in WB on Mouse:", "title": "Dopamine D2 receptor dysfunction is rescued by adenosine A2A receptor antagonism in a model of DYT1 dystonia.", "author": "Napolitano F, Pasqualetti M, Usiello A, Santini E, Pacini G, Sciamanna G, Errico F, Tassone A, Di Dato V, Martella G, Cuomo D, Fisone G, Bernardi G, Mandolesi G, Mercuri NB, Standaert DG, Pisani A.", "journal": "Neurobiol Dis. 2010 Jun;38(3):434-45."}, {"pmid": 21193245, "intro": "This antibody (previous batch) has been successfully used in IP on Rat:", "title": "Reduced expression of arrestin beta 2 by graft monocytes during acute rejection of rat kidneys.", "author": "Zakrzewicz A, Krasteva G, Wilhelm J, Dietrich H, Wilker S, Padberg W, Wygrecka M, Grau V.", "journal": "Immunobiology. 2011 Jul;216(7):854-61."}]

Goat Anti-Arrestin beta 2 Antibody

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