Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names Y5 receptor|NPYY5-R|NPY-Y5 receptor|NPY5-R|NPYR5|NPY5R|HGNC:7958|neuropeptide Y receptor Y5 | |
Usage Summary Immunoprecipitation: Customer feedback showed specific results when this product was used in IP. This antibody has been successfully used in IP: PMID: 30503694. | |
Accession ID NP_006165.1 | |
Blocking Peptide EBP06769 | |
Immunogen Peptide with sequence C-GLSNKENRLEEN, from the internal region of the protein sequence according to NP_006165.1. | |
Peptide Sequence C-GLSNKENRLEEN | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog | |
Reactive Species Human, Hamster | |
Human Gene ID 4889 | |
Mouse Gene ID 18168 | |
Rat Gene ID 25340 | |
Product Grade  | |
IHC Results In paraffin embedded Human Intestine shows staining of ganglion cells. Recommended concentration, 4-6µg/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:32000. | |
Western Blot Approx 50kDa band observed in Human Brain, Breast and Duodenum lysates (calculated MW of 50.7kDa according to NP_006165.1). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour. This antibody has been successfully used in WB on CHO-K1 cells: PMID: 30503694. This antibody has been successfully used in WB on Human: PMID: 28413811 and 20332211. | |
Application Type Pep-ELISA, WB, IP, IHC |
Goat Anti-Neuropeptide Y receptor Y5 Antibody
$464.00
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Selected References [{"pmid": 30503694, "intro": "This antibody has been successfully used in WB and IP on CHO-K1 cells:", "title": "Neuropeptide Y receptor interactions regulate its mitogenic activity", "author": "Magdalena Czarneckaa, Congyi Lua,b, Jennifer Ponsa, Induja Maheswarana, Pawel Ciborowskic, Lihua Zhangd, Amrita Cheemad, Joanna Kitlinskaa", "journal": "Neuropeptides. 2019 Feb;73:11-24. "}, {"pmid": 28413811, "intro": "This antibody has been successfully used in WB on Human:", "title": "Cell Surface Protein Detection to Assess Receptor Internalization.", "author": "Czarnecka M, Kitlinska J.", "journal": "Bio Protoc. 2016 Oct 20;6(20)"}, {"pmid": 33681186, "intro": "This antibody has been successfully used in WB on Human:", "title": "Neuropeptide Y/Y5 Receptor Pathway Stimulates Neuroblastoma Cell Motility Through RhoA Activation", "author": "Nouran Abualsaud, Lindsay Caprio, Susana Galli, Ewa Krawczyk, Lamia Alamri, Shiya Zhu, G Ian Kitlinska", "journal": "Front Cell Dev Biol. 2021 Feb 17;8:627090."}, {"pmid": 35484119, "intro": "This antibody has been successfully used in the following paper:", "title": "Hypoxia-activated neuropeptide Y/Y5 receptor/RhoA pathway triggers chromosomal instability and bone metastasis in Ewing sarcoma.", "author": "Congyi Lu et al.", "journal": "Nat Commun. 2022 Apr 28;13(1):2323."}, {"pmid": 20332211, "intro": "This antibody has been successfully used in WB on Human:", "title": "Neuropeptide Y Y5 receptor promotes cell growth through extracellular signal-regulated kinase signaling and cyclic AMP inhibition in a human breast cancer cell line.", "author": "Sheriff S, Ali M, Yahya A, Haider KH, Balasubramaniam A, Amlal H.", "journal": "Mol Cancer Res. 2010 Apr;8(4):604-14."}] |