Goat Anti-VPS11 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB06828
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
vacuolar protein sorting 11 homolog (S. cerevisiae)|vacuolar protein sorting protein 11|vacuolar protein sorting 11 (yeast homolog)|hVPS11|RNF108|PEP5|END1|HGNC:14583|vacuolar protein sorting 11 (yeast)|VPS11
Accession ID
NP_068375.3
Blocking Peptide
EBP06828
Immunogen
Peptide with sequence C-HALQEKDTQTKLE, from the internal region of the protein sequence according to NP_068375.3.
Peptide Sequence
C-HALQEKDTQTKLE
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Cow
Reactive Species
Human
Human Gene ID
55823
Mouse Gene ID
71732
Product Grade
IHC Results
In paraffin embedded Human Kidney shows brushborder staning in PCT cells and Human Pancreas shows cytoplasm staining of beta cells in islets of Langerhans. Recommended concentration, 3-6µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Preliminary experiments gave an approx 75kDa band in human duodenum, colon and placenta lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 108kDa according to NP_068375.3. The 75kDa band was successfully blocked by incubation with the immunizing peptide.
Application Type
Pep-ELISA, IHC

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