Goat Anti-OCT4 / POU5F1 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB06852
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
POU domain, class 5, transcription factor 1|OCT4|DADB-104B20.2|POU class 5 homeobox 1|octamer-binding transcription factor-3|POU-type homeodomain-containing DNA-binding protein|OTF3|OCT3|MGC22487|HGNC:9221|POU domain, class 5, transcription factor 1|OTF4|POU5F1
Accession ID
NP_002692.2; NP_976034.3
Blocking Peptide
EBP06852
Immunogen
Peptide with sequence C-VTTLGSPMHSN, from the C Terminus of the protein sequence according to NP_002692.2; NP_976034.3.
Product Comments
This antibody is expected to recognise both reported isoforms (NP_002692.2 and NP_976034.3)
Peptide Sequence
C-VTTLGSPMHSN
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog
Reactive Species
Human, Mouse
Human Gene ID
5460
Mouse Gene ID
18999
Rat Gene ID
294562
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:2000.
Western Blot
Approx 30kDa band observed in Human Placenta, and approx 28kDa in Mouse Heart lysates (calculated MW of 30.1kDa according to Human NP_976034.3 and 25.0kDa according to Mouse NP_001239381.1). Recommended concentration: 0.5-2µg/ml. Primary incubation was 1 hour
Application Type
Pep-ELISA, WB

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