Goat Anti-Rhophilin 2 (Internal) Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

RHPN2|p76RBE|rhophilin, Rho GTPase binding protein 2|rhophilin 2|rhophilin-2|RhoB effector|rhophilin-like protein|P76RBE|RHOBP|76 kDa RhoB effector protein|GTP-Rho-binding protein 2|rhophilin-like Rho-GTPase binding protein

NP_149094.3

EBP06853

Peptide with sequence C-TNKNRQKSAST, from the internal region of the protein sequence according to NP_149094.3.

C-TNKNRQKSAST

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Cow

Human

85415

In paraffin embedded Human Kidney shows staining of cellular membrane and cytoplasm in convoluted tubular cells. Recommended concentration 5-10µg/ml.

Antibody detection limit dilution 1:32000.

Preliminary experiments gave an approx 35kDa band in Human Pancreas and Prostate lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 77kDa according to NP_149094.2. The 35kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA, IHC