Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names OTTHUMP00000016224|OTTHUMP00000016223|HMGA1A|nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y|high-mobility group (nonhistone chromosomal) protein isoforms I and Y|OTTHUMP00000039618|MGC4854|MGC4242|MGC12816|HMGIY|HMG-R|HGNC:5010|high mobility group AT-hook 1|HMGA1 | |
Usage Summary Additional validation: This antibody has been successfully used in qRT-PCR on Human: Cello et al. (2013) PMID: 23545254. | |
Accession ID NP_665906.1; NP_665908.1; NP_002122.1; NP_665909.1; NP_665912.1; NP_665910.1 | |
Blocking Peptide EBP06859 | |
Immunogen Peptide with sequence C-LASKQEKDGTEK, from the internal region of the protein sequence according to NP_665906.1; NP_665908.1; NP_002122.1; NP_665909.1; NP_665912.1; NP_665910.1. | |
Product Comments This antibody is expected to recognise isoform a (also called HMG-I; NP_665906.1; NP_665908.1) and isoform b (also called HMG-Y; NP_002122.1; NP_665909.1; NP_665910.1; NP_665912.1). | |
Peptide Sequence C-LASKQEKDGTEK | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog, Pig, Cow | |
Reactive Species Human | |
Human Gene ID 3159 | |
Product Grade  | |
IHC Results Paraffin embedded Human Placenta and Small Intestine. Recommended concentration: 4µg/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:32000. | |
Western Blot Preliminary experiments gave an approx 50kDa band in Human Testis and Ovary lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 11.7kDa according to isoform a (also called HMG-I; NP_665906.1; NP_665908.1; NP_665911.1) and 10.7kDa according to isoform b( (also called HMG-Y; NP_002122.1; NP_665909.1; NP_665910.1; NP_665912.1). The 50kDa band was successfully blocked by incubation with the immunizing peptide. | |
Application Type Pep-ELISA, IHC |
Goat Anti-HMGA1 Antibody
$423.00
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Selected References [{"pmid": 23545254, "intro": "This antibody has been successfully used in qRT-PCR on Human:", "title": "Knockdown of HMGA1 inhibits human breast cancer cell growth and metastasis in immunodeficient mice.", "author": "Di Cello F, Shin J, Harbom K, Brayton C.", "journal": "Biochem Biophys Res Commun. 2013 Apr 26;434(1):70-4."}] |