Goat Anti-MMP9 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB06890
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
MMP9|matrix metalloproteinase 9|gelatinase B|92kDa gelatinase|92kDa type IV collagenase|HGNC:7176|CLG4B|GELB|92kD type IV collagenase|gelatinaseB|macrophage gelatinase|matrix metalloproteinase 9 (gelatinase B, 92kD gelatinase, 92kD type IV collagenase)|type V collagenase
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and HeLa cells. Recommended concentration: 10µg/ml.
Accession ID
NP_004985.2
Blocking Peptide
EBP06890
Immunogen
Peptide with sequence C-KLDSVFEEPLSKK, from the internal region of the protein sequence according to NP_004985.2.
Peptide Sequence
C-KLDSVFEEPLSKK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human, Mouse
Human Gene ID
4318
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Western Blot
Approx.80kDa and 70kDa bands observed in Human Peripheral Blood Mononucleocytes lysates. These bands correspond to the mature forms (http://www.uniprot.org/uniprot/P14780). This protein has a calculated MW of 78.5kDa according to NP_004985.2, but the precursor is usually seen as 92kDa or larger. Recommended concentration: 1-3µg/ml. Approx.75kDa band observed in Mouse Heart lysates (calculated MW of 80.5kDa according to Mouse NP_038627.1). Recommended concentration: 0.1-0.3µg/ml.
Application Type
Pep-ELISA, IF, WB

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