Goat Anti-TRPC6 Antibody

$423.00

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SKU: EB06901
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
TRP6|FSGS2|FLJ14863|FLJ11098|transient receptor potential channel 6|TRP6|HGNC:12338|transient receptor potential cation channel, subfamily C, member 6|TRPC6
Usage Summary
Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.
Accession ID
NP_004612.2
Blocking Peptide
EBP06901
Immunogen
Peptide with sequence C-EKLSMEPNQEETN, from the C Terminus of the protein sequence according to NP_004612.2.
Peptide Sequence
C-EKLSMEPNQEETN
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Dog, Pig, Cow
Reactive Species
Human
Human Gene ID
7225
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:2000.
Western Blot
Approx 100kDa band observed in lysates of cell line A549 (calculated MW of 106kDa according to NP_004612.2). Preliminary experiments also showed 100kDa in Human Lung lysates and in lysates of cell line HEK293.This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature. This antibody has been successfully used in WB on Mouse PMID: 32882106.
Application Type
Pep-ELISA, WB, FC
Selected References
[{"pmid": 32882106, "intro": "This antibody has been successfully used in WB on Mouse:", "title": "Implication of TRPC3 channel in gustatory perception of dietary lipids", "author": "Babar Murtaza, Aziz Hichami, Amira S Khan, Jiri Plesnik, Omar Sery, Alexander Dietrich, Lutz Birnbaumer, Naim A Khan", "journal": "Acta Physiol (Oxf). 2020 Sep 3;e13554.  doi: 10.1111/apha.13554."}]