Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size 100 µg
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names histamine H(1) receptor\|OTTHUMP00000160133\|histamine receptor, subclass H1\|hisH1\|H1-R\|HGNC:5182\|histamine receptor H1\|HRH1
Usage Summary Immunofluorescence: This product has been successfully used in IF on Rat (PMID: 30413645 and 30143981). Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
Accession ID NP_000852.1; NP_001091681.1; NP_001091682.1; NP_001091683.1
Blocking Peptide EBP06904
Immunogen Peptide with sequence CNENFKKTFKRILH, from the C Terminus of the protein sequence according to NP_000852.1; NP_001091681.1; NP_001091682.1; NP_001091683.1.
Product Comments Variants (NP_000852.1; NP_001091681.1; NP_001091682.1; NP_001091683.1) encode the same protein.
Peptide Sequence CNENFKKTFKRILH
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions Refrigerated
Predicted Species Human, Mouse, Rat, Dog, Cow
Reactive Species Human, Rat
Human Gene ID 3269
Mouse Gene ID 15465
Rat Gene ID 24448
Product Grade
IHC Results This product has been successfully used in IHC on Rat (PMID: 30226827).
ELISA Detection Limit Antibody detection limit dilution 1:32000.
Application Type Pep-ELISA, IHC, IF, FC

Selected References

[{"pmid": 30143981, "intro": "This antibody has been successfully used in IF on Rat:", "title": "Histamine Excites Rat GABAergic Ventral Pallidum Neurons via Co-activation of H1 and H2 Receptors", "author": "Miao-Jin Ji, Xiao-Yang Zhang, Xiao-Chun Peng, Yang-Xun Zhang, Zi Chen, Lei Yu, Jian-Jun Wang, Jing-Ning Zhu", "journal": "Neurosci Bull. 2018 Dec;34(6):1029-1036."}, {"pmid": 30226827, "intro": "This antibody has been successfully used in IHC on Rat:", "title": "Regularizing firing patterns of rat subthalamic neurons ameliorates parkinsonian motor deficits.", "author": "Zhuang QX, Li GY, Li B, Zhang CZ, Zhang XY, Xi K, Li HZ, Wang JJ, Zhu JN.", "journal": "J Clin Invest. 2018 Sep 18."}, {"pmid": 30413645, "intro": "This antibody has been successfully used in IF on Rat:", "title": "Histamine H1 Receptor Contributes to Vestibular Compensation.", "author": "Chen ZP, Zhang XY, Peng SY, Yang ZQ, Wang YB, Zhang YX, Chen X, Wang JJ, Zhu JN", "journal": "J Neurosci. 2019 Jan 16;39(3):420-433. "}]

Goat Anti-Histamine Receptor H1 (C Term) Antibody

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