Goat Anti-GOLGA6 / GLP Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

GOLGA6|golgi autoantigen, golgin subfamily a, member 6|HGNC:13567|GLP|Golgi autoantigen, golgin subfamily a, 6|Golgin linked to PML|golgin-like protein|GOLGA6B|GOLGA|Golgin subfamily A member 6B

NP_061122.4; NP_001033729.1

EBP06925

Peptide with sequence C-DLPKEKADGTEQ, from the internal region of the protein sequence according to NP_061122.4; NP_001033729.1.

GeneID 55889 may be a pseudogene, while GeneID 342096 is thought to encode a functional protein.

C-DLPKEKADGTEQ

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human

Human

55889, 342096

In paraffin embedded Human Cerebral Cortex shows localized cytoplasm staining consistent with Golgi. Recommended concentration, 3-6µg/ml.

Antibody detection limit dilution 1:1000.

Preliminary experiments gave an approx 45kDa band in human liver lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 79.9kDa according to NP_001033729.1. The 45kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA, IHC