Goat Anti-Duffy / FY / DARC Antibody

$464.00

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SKU: EB06940
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
HGNC:4035|CTA-134P22.3|plasmodium vivax receptor|glycoprotein D|duffy blood group chemokine receptor|Fy glycoprotein|Duffy blood group, chemokine receptor|Duffy blood group, atypical chemokine receptor|Duffy blood group antigen|Duffy antigen chemokine receptor|WBCQ1|GpFy|GPD|FY|Dfy|DARC|CD234|CCBP1|atypical chemokine receptor 1 (Duffy blood group)|ACKR1
Usage Summary
Flow cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml. A customer has reported successful staining of HEK293S-TetR transfected with DARC receptor at a dilution of 1/1000.
Accession ID
NP_001116423.1; NP_002027.2
Blocking Peptide
EBP06940
Immunogen
Peptide with sequence HRAELSPSTENSSQLDFED-C, from the N Terminus of the protein sequence according to NP_001116423.1; NP_002027.2.
Product Comments
This antibody is expected to recognize both reported isoforms (NP_001116423.1; NP_002027.2).
Peptide Sequence
HRAELSPSTENSSQLDFED-C
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
2532
Product Grade
IHC Results
In paraffin embedded Human Kidney stains epithelial cells of renal tubules and endothelial cells of vessels. The granular staining is compatible with the endoplasmic reticulum association of the protein. Recommended concentration: 3µg/ml. Paraffin embedded Human Lung. Recommended concentration: 5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Approx 37kDa band observed in Human Liver lysates (calculated MW of 35.6kDa according to NP_002027.2). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.
Application Type
Pep-ELISA, WB, IHC, FC