Goat Anti-PCK1 / PEPCKC (internal) Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

PEPCK-C|phosphopyruvate carboxylase|phosphoenolpyruvate carboxylase|cytosolic phosphoenolpyruvate carboxykinase 1|PEP carboxykinase|PEPCKC|PEPCK1|MGC22652|HGNC:8724|phosphoenolpyruvate carboxykinase 1 (soluble)|PCK1

NP_002582.3

EBP06943

Peptide with sequence C-HVNWFRKDKEGK, from the internal region of the protein sequence according to NP_002582.3.

C-HVNWFRKDKEGK

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Dog

Human

5105

Paraffin embedded Human Cerebral Cortex, Kidney and Liver. Recommended concentration: 2µg/ml.

Antibody detection limit dilution 1:8000.

Preliminary experiments gave bands at approx 70kDa, 45kDa and 28kDa in Human Adipose, Kidney and Liver lysates after 0.3 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the additional bands we observe given the calculated size of 69.2 kDa according to NP_002582.2. All three detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

Pep-ELISA, IHC