Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
| |
Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names heterochromatin protein 1-alpha|heterochromatin protein 1 homolog alpha|antigen p25|HP1A|Heterochromatin protein-1|chromobox homolog 5 (Drosophila HP1 alpha)|HP1Hs-alpha|HP1|HGNC:1555|chromobox homolog 5 (HP1 alpha homolog, Drosophila)|HP1-ALPHA|CBX5 | |
Usage Summary Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10µg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. | |
Accession ID NP_036249.1; NP_001120793.1; NP_001120794.1 | |
Blocking Peptide EBP06957 | |
Immunogen Peptide with sequence C-NKRKSNFSNSADDIK, from the internal region of the protein sequence according to NP_036249.1; NP_001120793.1; NP_001120794.1. | |
Product Comments Variants (NP_036249.1; NP_001120793.1; NP_001120794.1) encode the same protein. | |
Peptide Sequence C-NKRKSNFSNSADDIK | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Dog | |
Reactive Species Human | |
Human Gene ID 23468 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:128000. | |
Western Blot Approx 24-25kDa band observed in lysates of cell lines A43N and K562 (calculated MW of 22.2kDa according to Human NP_036249.1). Recommended concentration: 0.03-0.1µg/ml. Primary incubation 1 hour at room temperature. | |
Application Type Pep-ELISA, WB, IF, FC |
Goat Anti-CBX5 / HP1-Alpha Antibody
$464.00
| SKU | Unit Size | Price |
|---|---|---|
Select a unit size:
Selected References [{"pmid": 30377371, "intro": "This antibody (previous batch) has been successfully used in the following paper:", "title": "A high-throughput pipeline for validation of antibodies", "author": "Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen", "journal": "Nat Methods. 2018 Nov;15(11):909-912"}] |