Goat Anti-EGFR Antibody

$423.00

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SKU: EB06962
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
EGFR|epidermal growth factor receptor|erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian|HGNC:3236|ERBB|ERBB1|mENA|avian erythroblastic leukemia viral (v-erb-b) oncogene homolog|epidermal growth factor receptor|epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b) oncogene homolog)|truncated epidermal growth factor receptor
Usage Summary
Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Accession ID
NP_005219.2
Blocking Peptide
EBP06962
Immunogen
Peptide with sequence C-QKGSHQISLDNPD, from the internal region of the protein sequence according to NP_005219.2.
Product Comments
This antibody is expected to recognise isoform a (NP_005219.2) only.
Peptide Sequence
C-QKGSHQISLDNPD
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
1956
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 170kDa band observed in lysates of cell line HeLa (calculated MW of 134kDa according to NP_005219.2). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.1-0.3µg/ml.
Application Type
Pep-ELISA, WB
Selected References
[{"pmid": 30377371, "intro": "This antibody has been successfully used in the following paper:", "title": "A high-throughput pipeline for validation of antibodies", "author": "Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen", "journal": "Nat Methods. 2018 Nov;15(11):909-912"}]