Goat Anti-MDM2 (isoform) Antibody

$423.00

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SKU: EB06990
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
MDM2|Mdm2|transformed 3T3 cell double minute 2|p53 binding protein (mouse)|HGNC:6973|MGC71221|hdm2|mouse double minute 2 homolog|mouse double minute 2, human homolog of|p53-binding protein|p53-binding protein MDM2|ubiquitin-protein ligase E3 Mdm2|Mdm2 p53 binding protein homolog (mouse)|HDMX|MGC5370|Mdm2, transformed 3T3 cell double minute 2, p53 binding protein|OTTHUMP00000183495|double minute 2, human homolog of
Accession ID
NP_002383.2
Blocking Peptide
EBP06990
Immunogen
Peptide with sequence C-DELSGERQRKRHKSD, from the internal region of the protein sequence according to NP_002383.2.
Product Comments
This antibody is expected to recognize isoform MDM2 (NP_002383.2) only.
Peptide Sequence
C-DELSGERQRKRHKSD
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Rat, Cat, Dog, Pig, Cow
Reactive Species
Human
Human Gene ID
4193
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Western Blot
Approx 55kDa band observed in Human Liver lysates (calculated MW of 56.0kDa according to NP_002383.2). In transfected HEK293 transiently expressing MDM2 bands of approx. 48kDa, 60KDa, 70kDa and 90kDa are observed. These bands are not observed in the non-transfected HEK293. The 80kDa band is endogenously epressed in the negative control. Recommended concentration: 0.3-1.0µg/ml.
Application Type
Pep-ELISA, WB