Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names sonic hedgehog|SMMCI|HPE3|HLP3|HHG1|HGNC:10848|sonic hedgehog homolog (Drosophila)|SHH | |
Usage Summary Immunofluorescence: Strong expression of the protein seen in the membranes of HeLa, U251 and NIH3T3 cells. Recommended concentration: 10µg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. | |
Accession ID NP_000184.1 | |
Blocking Peptide EBP07032 | |
Immunogen Peptide with sequence C-DSEALHPLGMAVK, from the C Terminus of the protein sequence according to NP_000184.1. | |
Peptide Sequence C-DSEALHPLGMAVK | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Rat, Dog | |
Reactive Species Human, Mouse | |
Human Gene ID 6469 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:32000. | |
Western Blot Approx 48kDa band observed in Human Liver lysates (calculated MW of 49.6kDa according to NP_000184.1 Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat Liver for this particular batch. | |
Application Type Pep-ELISA, WB, IF, FC |
Goat Anti-SHH Antibody
$464.00
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