Goat Anti-GAPDH (Internal), Biotinylated Antibody

$423.00

Category: Primary Antibodies
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SKU: EB07069-B
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
MGC88685|HGNC:4141|glyceraldehyde 3-phosphate dehydrogenase|peptidyl-cysteine S-nitrosylase GAPDH|epididymis secretory sperm binding protein Li 162eP|aging-associated gene 9 protein|HEL-S-162eP|GAPD|G3PD|glyceraldehyde-3-phosphate dehydrogenase|GAPDH
Accession ID
NP_002037.2; NP_001243728.1
Blocking Peptide
EBP07069-B
Immunogen
Peptide with sequence C-GVNHEKYDNSLK., from the internal region of the protein sequence according to NP_002037.2; NP_001243728.1.
Product Comments
This antibody is expected to recognize both reported isoforms (NP_002037.2; NP_001243728.1). Reported variants represent identical protein: NP_001276674.1, NP_002037.2, NP_001276675.1. GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.
Peptide Sequence
C-GVNHEKYDNSLK.
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human, Mouse, Rat
Human Gene ID
2597
Mouse Gene ID
14433
Rat Gene ID
24383
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Approx 36kDa band observed in Human Liver lysates (calculated MW of 36.1kDa according to NP_002037.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 1-3µg/ml.
Application Type
Pep-ELISA, WB

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