Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size 100 µg
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names adenosine receptor subtype A2a\|adenosine A2 receptor\|hA2aR\|RDC8\|ADORA2\|HGNC:263\|adenosine A2a receptor\|ADORA2A
Usage Summary Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 cells. Recommended concentration: 10µg/ml.
Accession ID NP_000666.2
Blocking Peptide EBP07079
Immunogen Peptide with sequence HELKGVCPEPPGLDD, from the C Terminus of the protein sequence according to NP_000666.2.
Peptide Sequence HELKGVCPEPPGLDD
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions Refrigerated
Predicted Species Human
Reactive Species Human, Mouse, Macaque
Human Gene ID 135
Product Grade
ELISA Detection Limit Antibody detection limit dilution 1:8000.
Western Blot This antibody has been successfully used in Western blot on Macaque: van der Putten C et al. (2009) PMID: 19494284. This antibody has been successfully used in Western blot on Mouse: He X et al. (2013) PMID: 23712033, and Zhao L et al. (2015) PMID: 26171727.
Application Type Pep-ELISA, WB, IF

Selected References

[{"pmid": 23712033, "intro": "This antibody has been successfully used in the WB on Mouse:", "title": "A feedback loop in PPAR?-adenosine A2A receptor signaling inhibits inflammation and attenuates lung damages in a mouse model of LPS-induced acute lung injury.", "author": "He X, Hu JL, Li J, Zhao L, Zhang Y, Zeng YJ, Dai SS, He FT.", "journal": "Cell Signal. 2013 Sep;25(9):1913-23."}, {"pmid": 26171727, "intro": "This antibody has been successfully used in WB on Mouse:", "title": "The mutual regulation between miR-214 and A2AR signaling plays an important role in inflammatory response.", "author": "Zhao L, Liu YW, Yang T, Gan L, Yang N, Dai SS, He F.", "journal": "Cell Signal. 2015 Oct;27(10):2026-34."}, {"pmid": 19494284, "intro": "This antibody has been successfully used in WB on Macaque:", "title": "Differential expression of adenosine A3 receptors controls adenosine A2A receptor-mediated inhibition of TLR responses in microglia.", "author": "van der Putten C, Zuiderwijk-Sick EA, van Straalen L, de Geus ED, Boven LA, Kondova I, IJzerman AP, Bajramovic JJ.", "journal": "J Immunol. 2009 Jun 15;182(12):7603-12."}]

Goat Anti-Adenosine A2a Receptor Antibody

Related products