Goat Anti-CD14 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB07103
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
HGNC:1628|CD14 antigen|CD14
Usage Summary
Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.
Accession ID
NP_000582.1
Blocking Peptide
EBP07103
Immunogen
Peptide with sequence C-KRVDADADPRQYAD, from the internal region of the protein sequence according to NP_000582.1.
Peptide Sequence
C-KRVDADADPRQYAD
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
929
Product Grade
IHC Results
Paraffin embedded Human Tonsil. Recommended concentration: 6-8µg/ml. Weak positive staining of Non germinal center cells in Human Lymph Node was also observed.
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 55kDa band observed in Human Lymph node and Tonsil lysates (calculated MW of 40.1kDa according to NP_000582.1).The observed molecular weight corresponds to the glycosylated form, and was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IHC, FC

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