Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names zinc finger CCCH-type domain containing 1|zinc finger CCCH type domain containing 1|ZC3HDC1|ZC3H1|PARP-12|FLJ22693|HGNC:21919|poly (ADP-ribose) polymerase family, member 12|PARP12 | |
Usage Summary Immunofluorescence: Anonymous customer has confirmed specificity by siRNA-mediated PARP2 knockdown. | |
Accession ID NP_073587.1 | |
Blocking Peptide EBP07110 | |
Immunogen Peptide with sequence C-NAHDIKNKSSAP, from the internal region of the protein sequence according to NP_073587.1. | |
Peptide Sequence C-NAHDIKNKSSAP | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Dog | |
Reactive Species Human | |
Human Gene ID 64761 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:128000. | |
Western Blot Preliminary experiments gave an approx 110kDa band in Human Liver lysates and in lysates of hepatoblastoma cell line HEPG2 after 0.03µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 79.1kDa according to NP_073587.1. The 110kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? | |
Application Type Pep-ELISA, IF |
Goat Anti-PARP12 Antibody
$423.00
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