Goat Anti-Amphiphysin / AMPH Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

AMPH|amphiphysin|amphiphysin (Stiff-Man syndrome with breast cancer 128kDa autoantigen)|HGNC:471|AMPH1

NP_001626.1; NP_647477.1

EBP07245

Peptide with sequence C-KRKDESRISKAE, from the internal region of the protein sequence according to NP_001626.1; NP_647477.1.

This antibody is expected to recognise both reported isoforms (NP_001626.1 and NP_647477.1).

C-KRKDESRISKAE

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Dog

Human, Mouse

273

218038

In paraffin embedded Human Cerebellum shows nuclear and fibrous staning. Recommended concentration, 2-4µg/ml.

Antibody detection limit dilution 1:16000.

Approx 125kDa band observed in mouse brain lysates (calculated MW of 76.3kDa according to NP_001626.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Wigge et al, Mol Biol Cell. 1997 Oct;8(10):2003-15.; PMID: 9348539). Recommended concentration: 0.1-0.5µg/ml. A minor band of unknown identity was also consistently observed at 45kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA, WB, IHC