Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names WNT-3 proto-oncogene protein precursor|MGC138323|MGC138321|MGC131950|INT4|wingless-type MMTV integration site family, member 3|WNT3 | |
Accession ID NP_110380.1 | |
Blocking Peptide EBP07253 | |
Immunogen Peptide with sequence CGRGHNTRTEKRKEK, from the internal region of the protein sequence according to NP_110380.1. | |
Peptide Sequence CGRGHNTRTEKRKEK | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog | |
Reactive Species Human | |
Human Gene ID 7473 | |
Mouse Gene ID 22415 | |
Rat Gene ID 24882 | |
Product Grade  | |
IHC Results In paraffin embedded Human Breast shows cytoplasm staining in some luminal epithelial cells. Recommended concentration: 2-4µg/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:16000. | |
Western Blot Approx 40kDa band observed in lysates of cell line HEK293 (calculated MW of 39.6kDa according to NP_110380.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour. | |
Application Type Pep-ELISA, WB, IHC |
Goat Anti-WNT3 Antibody
$423.00
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Selected References [{"pmid": 24482229, "intro": "This antibody (previous batch) has been successfully used in IHC on Mouse:", "title": "Wnt protein-mediated satellite cell conversion in adult and aged mice following voluntary wheel\r\nrunning.", "author": "Fujimaki S, Hidaka R, Asashima M, Takemasa T, Kuwabara T.", "journal": "J Biol Chem. 2014 Mar 14;289(11):7399-412."}, {"pmid": 21746862, "intro": "This antibody (previous batch) has been successfully used in ICC/IF on Mouse:", "title": "Reduction in paracrine Wnt3 factors during aging causes impaired adult neurogenesis.", "author": "Okamoto M, Inoue K, Iwamura H, Terashima K, Soya H, Asashima M, Kuwabara T.", "journal": "FASEB J. 2011 Jul 11."}, {"pmid": 27226528, "intro": "This antibody (previous batch) has been successfully used in IHC on Rat:", "title": "Diabetes Impairs Wnt3 Protein-induced Neurogenesis in Olfactory Bulbs via Glutamate Transporter 1Inhibition.", "author": "Wakabayashi T, Hidaka R, Fujimaki S, Asashima M, Kuwabara T.", "journal": "J Biol Chem. 2016 Jul 15;291(29)"}] |