Goat Anti-HSPA8 (Isoform 1) Antibody

$464.00

Category: Primary Antibodies
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SKU: EB07295
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
uncharacterized bone marrow protein BM034|lipopolysaccharide-associated protein|71-kDa|heat shock cognate protein|heat shock cognate protein 54|heat shock 70kd protein 10|heat shock 70kD protein 8|constitutive heat shock protein 70|N-myristoyltransferase inhibitor protein 71|LPS-associated protein 1|NIP71|MGC29929|MGC131511|LAP|HSPA10|HSP73|HSP71|HSC71|HSC70|HSC54|heat shock 70kDa protein 8|HSPA8
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10µg/ml
Accession ID
NP_006588.1
Blocking Peptide
EBP07295
Immunogen
Peptide with sequence C-EKLQGKINDEDKQK, from the internal region of the protein sequence according to NP_006588.1.
Product Comments
This antibody is expected to recognise isoform 1 (NP_006588.1) only.
Peptide Sequence
C-EKLQGKINDEDKQK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog
Reactive Species
Human, Mouse
Human Gene ID
3312
Mouse Gene ID
15481
Rat Gene ID
24468
Product Grade
IHC Results
Paraffin embedded Human Prostate. Recommended concentration: 5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:4000.
Western Blot
Approx 75kDa band observed in lysates of cell lines HepG2 and NIH3T3 (calculated MW of 70.9kDa according to Human NP_006588.1 and Mouse NP_112442.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.03-0.1µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, IHC

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