Goat Anti-Semaphorin 5A Antibody

$423.00

Category: Primary Antibodies
SKU Unit Size Price

Select a unit size:

SKU: EB07352
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane|semF|SEMAF|HGNC:10736|sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A|SEMA5A
Accession ID
NP_003957.1
Blocking Peptide
EBP07352
Immunogen
Peptide with sequence DEATKKACYSKGKS, from the internal region of the protein sequence according to NP_003957.1.
Product Comments
The immunizing peptide corresponds to a part of the extracellular domain.
Peptide Sequence
DEATKKACYSKGKS
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Cow
Reactive Species
Human
Human Gene ID
9037
Mouse Gene ID
20356
Rat Gene ID
310207
Product Grade
IHC Results
In paraffin embedded Human Kidney shows membanous and vesicular staining in PCT cells. Recommended concentration: 4-6µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Preliminary experiments gave an approx 90kDa in Mouse Brain lysates and approx 25kDa band in Human Brain (Cerebellum), Colon, Heart and Kidney lysates after 0.03µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 121kDa according to NP_003957.1. The 90kDa and 25kDa band were successfully blocked by incubation with the immunizing peptide.
Application Type
Pep-ELISA, IHC

Related products