Goat Anti-DSCAM Antibody

$464.00

Category: Primary Antibodies
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SKU: EB07370
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
human CHD2-52 down syndrome cell adhesion molecule|CHD2-52|CHD2-42|Down syndrome cell adhesion molecule|DSCAM
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the nuclei of MCF7 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_001380.2; NP_001258463.1
Blocking Peptide
EBP07370
Immunogen
Peptide with sequence C-DSWDSAQRTKDVSPQ, from the internal region of the protein sequence according to NP_001380.2; NP_001258463.1.
Product Comments
This antibody is expected to recognise both reported isoforms (NP_001380.2 and NP_996770.1).
Peptide Sequence
C-DSWDSAQRTKDVSPQ
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog
Reactive Species
Human
Human Gene ID
1826
Mouse Gene ID
13508
Rat Gene ID
171119
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Approx 200-220kDa band observed in HepG2 cell lysates (calculated MW of 174kDa according to NP_996770.1 ). This molecular weight is routinely observed by other sources. Recommended concentration: 2-3µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, FC

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