Goat Anti-FEZ1 / Zygin 1 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB07508
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
fasciculation and elongation protein zeta 1 (zygin I)|zygin 1|FEZ1
Accession ID
NP_005094.1
Blocking Peptide
EBP07508
Immunogen
Peptide with sequence C-QNKQKEQRELMKK, from the internal region of the protein sequence according to NP_005094.1.
Product Comments
This antibody is expected to recognize isoform 1 (NP_005094.1) only.
Peptide Sequence
C-QNKQKEQRELMKK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog
Reactive Species
Human, Rat
Human Gene ID
9638
Mouse Gene ID
235180
Rat Gene ID
81730
Product Grade
IHC Results
Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5-8µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Western Blot
Approx 75kDa band observed in lysates of cell line Kelly, ans in Rat Brain lysates (calculated MW of 45.1kDa according to human NP_005094.1 and 45.2kDa according to Rat NP_112328.1). This molecular weight is routinely observed by other sources and was successfully blocked by incubation with the immunising peptide. Recommended concentration:0.1-0.3g/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IHC

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