Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size 100 µg
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names AW106902\|cholecystokinin A receptor\|Cckar
Accession ID NP_033957.1
Blocking Peptide EBP07509
Immunogen Peptide with sequence C-KFDASQKKSAKEKR, from the internal region of the protein sequence according to NP_033957.1.
Peptide Sequence C-KFDASQKKSAKEKR
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions Refrigerated
Predicted Species Mouse, Rat
Reactive Species Rat
Mouse Gene ID 12425
Rat Gene ID 24889
Product Grade
ELISA Detection Limit Antibody detection limit dilution 1:64000.
Western Blot Approx. 75kDa band observed in purified membranes of Rat insulinoma cell line RIN14B (calculated MW of 48.4kDa according to NP_033957.1). Data were published by El-Kouhen K and Morisset J. Int J Endocrinol. 2009;2009:875167; PMID: 20011057. The observed molecular weight also corresponds to earlier findings in literature with different antibodies (Morisset et al J Histochem Cytochem. 2003 Nov;51(11):1501-13; PMID: 14566022). Recommended concentration: 0.1-0.3µg/ml.
Application Type Pep-ELISA, WB

Selected References

[{"pmid": 19959964, "intro": "This antibody has been successfully used in IF on Rat:", "title": "Control of somatostatin (SS) secretion by CCK-1 and CCK-2 receptors' occupation in RIN-14B cells, a rat pancreatic islet cell line.", "author": "El-Kouhen K, Morisset J.", "journal": "Pancreas. 2010 Mar;39(2):127-34."}, {"pmid": 20011057, "intro": "This antibody has been successfully used in WB on Rat:", "title": "Cholecystokinin and Somatostatin Negatively Affect Growth of the Somatostatin-RIN-14B Cells.", "author": "El-Kouhen K, Morisset J.", "journal": "Int J Endocrinol. 2009; 875167."}]

Goat Anti-CCKA Receptor (mouse) Antibody

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