Goat Anti-PRPF31 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB07513
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
pre-mRNA processing factor 31 homolog (yeast)|pre-mRNA processing factor 31 homolog|PRP31 pre-mRNA processing factor 31 homolog (yeast)|RP11|PRP31|NY-BR-99|DKFZp566J153|PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae)|PRPF31
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of HEK293 and HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_056444.3
Blocking Peptide
EBP07513
Immunogen
Peptide with sequence KELGNSLDKCKNNEN, from the internal region of the protein sequence according to NP_056444.3.
Peptide Sequence
KELGNSLDKCKNNEN
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Pig, Cow
Reactive Species
Human
Human Gene ID
26121
Mouse Gene ID
68988
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 60kDa band observed in nuclear lysates of cell lines A431, HeLa, HEK293 and NIH3T3 (calculated MW of 55.4kDa according to Human NP_056444.3 and Mouse NP_081604.3). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, FC

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