Goat Anti-Farnesoid X receptor / FXR Antibody

$423.00

Category: Primary Antibodies
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SKU: EB07540
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
farnesol receptor HRR-1|RIP14|HRR-1|FXR|BAR|nuclear receptor subfamily 1, group H, member 4|NR1H4|HRR1|farnesoid X receptor
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of U2OS cells. Recommended concentration: 10ug/ml.

Accession ID
NP_005114.1; NP_001193906.1; NP_001193922.1; NP_001193921.1; NP_001193907.1
Blocking Peptide
EBP07540
Immunogen
Peptide with sequence KSCREKTELTPDQQ, from the internal region of the protein sequence according to NP_005114.1; NP_001193906.1; NP_001193922.1; NP_001193921.1; NP_001193907.1.
Peptide Sequence
KSCREKTELTPDQQ
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Cow
Reactive Species
Human
Human Gene ID
9971
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Preliminary testing showed a band at approx 55kDa in Human Kidney, Colon, Duodenum and Ileum lysate after 0.5µg/ml antibody staining (calculated MW of 54.4kDa according to NP_005114.1). Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, IF, FC

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