Goat Anti-ITCH / AIF4 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB07645
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
ubiquitin protein ligase ITCH|itchy homolog E3 ubiquitin protein ligase|dJ468O1.1 (atrophin 1 interacting protein 4, AIP4)|atrophin-1 interacting protein 4|NFE2-associated polypeptide 1|dJ468O1.1|NAPP1|AIP4|itchy homolog E3 ubiquitin protein ligase (mouse)|AIF4|ITCH
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Accession ID
NP_113671.3; NP_001244066.1; NP_001244067.1
Blocking Peptide
EBP07645
Immunogen
Peptide with sequence C-EIKSHDLKPNGGN, from the internal region of the protein sequence according to NP_113671.3; NP_001244066.1; NP_001244067.1.
Peptide Sequence
C-EIKSHDLKPNGGN
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Cow
Reactive Species
Human
Human Gene ID
83737
Mouse Gene ID
16396
Rat Gene ID
311567
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Approx 90kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 86.4kDa according to NP_001244067.1) . Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, FC

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