Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names zinc finger, DHHC domain like containing 1|zinc finger, DHHC domain containing 8|membrane-associated DHHC8 zinc finger protein|ZNF378|ZDHHCL1|zinc finger, DHHC-type containing 8|ZDHHC8 | |
Accession ID NP_037505.1 | |
Blocking Peptide EBP07652 | |
Immunogen Peptide with sequence C-QRDHPQLKTPPSK, from the internal region of the protein sequence according to NP_037505.1. | |
Product Comments This antibody is expected to recognise isoform 2 (NP_037505.1) only. | |
Peptide Sequence C-QRDHPQLKTPPSK | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog, Pig | |
Reactive Species Human | |
Human Gene ID 29801 | |
Mouse Gene ID 27801 | |
Rat Gene ID 303796 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:16000. | |
Western Blot Approx 80-85kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 81.4kDa according to NP_037505.1). Recommended concentration: 1-3µg/ml.
An additional band of 48kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot. | |
Application Type Pep-ELISA, WB |
Goat Anti-ZDHHC8 Antibody
$423.00
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Selected References [{"pmid": 35150145, "intro": "This antibody has been successfully used in Western blot on Mouse:", "title": "Ppt1-deficiency dysregulates lysosomal Ca++ - homeostasis contributing to pathogenesis in a mouse model of CLN1 disease", "author": "Avisek Mondal, Abhilash P. Appu, Tamal Sadhukhan, Maria B. Bagh, Rafael M. Previde, Sriparna Sadhukhan, Stanko Stojilkovic, Aiyi Liu, Anil B. Mukherjee", "journal": "J Inherit Metab Dis. 2022 Feb 12. doi: 10.1002/jimd.12485."}] |