Goat Anti-Neuroligin 2 Antibody

$464.00

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SKU: EB07663
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
NLGN2|KIAA1366|neuroligin 2
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the mitochondria/cytoplasm of U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_065846.1
Blocking Peptide
EBP07663
Immunogen
Peptide with sequence C-NPPDTDIRDPGKK, from the internal region of the protein sequence according to NP_065846.1.
Peptide Sequence
C-NPPDTDIRDPGKK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog
Reactive Species
Human
Human Gene ID
57555
Mouse Gene ID
216856
Rat Gene ID
117096
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:4000.
Western Blot
Approx 90kDa band observed in Human Pancrease lysates (calculated MW of 90.8kDa according to NP_065846.1). Recommended concentration: 2-3µg/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Mouse and Rat Brain for this particular batch.
Application Type
Pep-ELISA, WB, IF, FC
Selected References
[{"pmid": 19827159, "intro": "This antibody has been successfully used in the following paper:", "title": "SynCAM1 expression correlates with restoration of central synapses on spinal motoneurons after two different models of peripheral nerve injury.", "author": "Zelano J, Berg A, Thams S, Hailer NP, Cullheim S.", "journal": "J Comp Neurol. 2009 Dec 10;517(5):670-82."}]