Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size 100 µg
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names arginase 1 liver\|AI type I arginase\|arginase 1\|Arg1
Usage Summary Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 cells. Recommended concentration: 10µg/ml.
Accession ID NP_058830.2
Blocking Peptide EBP07707
Immunogen Peptide with sequence C-NHKPETDYLKPPK, from the C Terminus of the protein sequence according to NP_058830.2.
Peptide Sequence C-NHKPETDYLKPPK
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions Refrigerated
Predicted Species Mouse, Rat
Reactive Species Human, Mouse, Rat
Mouse Gene ID 11846
Rat Gene ID 29221
Product Grade
ELISA Detection Limit Antibody detection limit dilution 1:1000.
Western Blot Approx 37kDa band observed in Mouse and Rat Liver lysates (calculated MW of 35kDa according to Rat NP_058830.2 and 34.8kDa according to Mouse NP_031508.1). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.
Application Type Pep-ELISA, WB, IF

Selected References

[{"pmid": 21497500, "intro": "This antibody (previous batch) has been successfully used in the following paper:", "title": "All-trans retinoic acid modifies the expression of clock and disease marker genes.", "author": "Sherman H, Gutman R, Chapnik N, Meylan J, le Coutre J, Froy O.", "journal": "J Nutr Biochem. 2011 Apr 14."}, {"pmid": 21352949, "intro": "This antibody (previous batch) has been successfully used in the following paper:", "title": "Caffeine alters circadian rhythms and expression of disease and metabolic markers.", "author": "Sherman H, Gutman R, Chapnik N, Meylan J, le Coutre J, Froy O.", "journal": "Int J Biochem Cell Biol. 2011 May;43(5):829-38."}]

Goat Anti-Arginase, type 1 / arg1(rat) Antibody

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