Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names uncoupling protein-2|uncoupling protein 2|UCPH|SLC25A8|uncoupling protein 2 (mitochondrial, proton carrier)|UCP2 | |
Usage Summary Immunofluorescence: Strong expression of the protein seen in the Mitochondria of MCF7 cells. Recommended concentration: 10µg/ml. | |
Accession ID NP_003346.2 | |
Blocking Peptide EBP07716 | |
Immunogen Peptide with sequence C-DSVKQFYTKGSEH, from the internal region of the protein sequence according to NP_003346.2. | |
Peptide Sequence C-DSVKQFYTKGSEH | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog | |
Reactive Species Human, Mouse, Rat | |
Human Gene ID 7351 | |
Mouse Gene ID 22228 | |
Rat Gene ID 54315 | |
Product Grade  | |
IHC Results Weak positive staining of red and white pulp cells in parafin embedded Human Kidney, at 6-7ug/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:128000. | |
Western Blot Approx 37kDa band observed in Rat Adipose and 28-30kDa in Mouse Spleen lysates (calculated MW of 33.4kDa according to Mouse NP_035801.3 and 33.4kDa according to Rat NP_062227.2). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature. | |
Application Type Pep-ELISA, WB, IF |
Goat Anti-Uncoupling protein 2 / UCP2 Antibody
$423.00
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Selected References [{"pmid": 23434936, "intro": "This antibody (previous batch) has been successfully used in IF/ICC in Mouse :", "title": "UCP2 Regulates the Glucagon Response to Fasting and Starvation.", "author": "Allister EM, Robson-Doucette CA, Prentice KJ, Hardy AB, Sultan S, Gaisano HY, Kong D, Gilon P, Herrera PL, Lowell BB, Wheeler MB.", "journal": "Diabetes. 2013 Feb 22. [Epub ahead of print]"}] |