Goat Anti-Robo3 (mouse) Antibody

$255.00

Category: Primary Antibodies
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SKU: EB07774
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
Robo3|roundabout homolog 3 (Drosophila)|Rbig1|RIG1|Rig-1|Robo3a|Robo3b|retinoblastoma inhibiting gene 1
Accession ID
NP_035378.1; AAD11628.1
Blocking Peptide
EBP07774
Immunogen
Peptide with sequence C-RRQKQRKELSHYT, from the internal region of the protein sequence according to NP_035378.1; AAD11628.1.
Product Comments
Both variants represent identical protein
Peptide Sequence
C-RRQKQRKELSHYT
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Mouse, Rat
Mouse Gene ID
19649
Rat Gene ID
315564
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Preliminary experiments gave an approx 75-80kDa band in Mouse Brain and Rat Brain lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 143kDa according to NP_035378.1and AAD11628.1. The 75-80kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Application Type
Pep-ELISA

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