Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size 100 µg
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names nuclear factor of activated T-cells, 45-kDa\|interleukin enhancer binding factor 2\|PRO3063\|MGC8391\|interleukin enhancer binding factor 2, 45kDa\|NF45\|ILF2
Accession ID NP_004506.2; NP_001254738.1
Blocking Peptide EBP07784
Immunogen Peptide with sequence C-EKAYEKPPEKKEG, from the internal region (near the C Terminus) of the protein sequence according to NP_004506.2; NP_001254738.1.
Product Comments This antibody is expected to recognize both reported isoforms (NP_004506.2; NP_001254738.1).
Peptide Sequence C-EKAYEKPPEKKEG
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions Refrigerated
Predicted Species Human, Mouse, Rat, Dog
Reactive Species Human
Human Gene ID 3608
Mouse Gene ID 67781
Rat Gene ID 310612
Product Grade
ELISA Detection Limit Antibody detection limit dilution 1:4000.
Western Blot Approx 43kDa band observed in Human Tonsil and Thymus lysates (calculated MW of 43.1kDa according to NP_004506.2). Recommended concentration: 0.03-0.1µg/ml. This antibody has been successfully used in WB in the following papers: Ye J et al, RNA. 2017 Aug;23(8):1270-1284. PMID: 28487382. And Shamanna RA et al, Oncogene. 2013 Oct 24;32(43):5176-85 PMID: 23208500.
Application Type Pep-ELISA, WB

Selected References

[{"pmid": 21969602, "intro": "This antibody (previous batch) has been successfully used in WB and IP on HeLa cells:", "title": "The NF90/NF45 complex participates in DNA break repair via nonhomologous end joining.", "author": "Shamanna RA, Hoque M, Lewis-Antes A, Azzam EI, Lagunoff D, Pe'ery T, Mathews MB.", "journal": "Mol Cell Biol. 2011 Dec;31(23):4832-43."}, {"pmid": 28487382, "intro": "This antibody has been successfully used in WB and IP on ESCs:", "title": "NF45 and NF90/NF110 coordinately regulate ESC pluripotency and differentiation", "author": "Ye J, Jin H, Pankov A, Song J, Blelloch R.", "journal": "RNA. 2017 Aug;23(8):1270-1284. "}, {"pmid": 23208500, "intro": "This antibody has been successfully used in WB on Human:", "title": "Induction of p53, p21 and apoptosis by silencing the NF90/NF45 complex in human papilloma virus-transformed cervical carcinoma cells.", "author": "Shamanna RA, Hoque M, Pe'ery T, Mathews MB.", "journal": "Oncogene. 2013 Oct 24;32(43):5176-85"}]

Goat Anti-ILF2 / NF45 Antibody

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