Goat Anti-Gabpb2 (mouse) Antibody

$255.00

Category: Primary Antibodies
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SKU: EB07819
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
Gabpb2|GA binding protein transcription factor, beta 2
Accession ID
NP_766100.1; NP_084161.1
Blocking Peptide
EBP07819
Immunogen
Peptide with sequence C-KSGRTTEPHTN, from the C Terminus of the protein sequence according to NP_766100.1; NP_084161.1.
Product Comments
This antibody is expected to recognise both reported isoforms (NP_766100.1; NP_084161.1).
Peptide Sequence
C-KSGRTTEPHTN
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Mouse, Rat
Mouse Gene ID
213054
Rat Gene ID
295263
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Preliminary experiments gave an approx 35kDa band in Rat Kidney lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 45.7kDa according to NP_766100.1 and NP_084161.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Application Type
Pep-ELISA

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