Goat Anti-NCF4 / P40PHOX Antibody

$423.00

Category: Primary Antibodies
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SKU: EB07895
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
neutrophil cytosolic factor 4 (40kD)|neutrophil cytosol factor 4|neutrophil NADPH oxidase factor 4|OTTHUMP00000043756|OTTHUMP00000028736|SH3PXD4|NCF|MGC3810|CTA-833B7.1|neutrophil cytosolic factor 4, 40kDa|P40PHOX|NCF4
Accession ID
NP_000622.2; NP_038202.1
Blocking Peptide
EBP07895
Immunogen
Peptide with sequence C-KDFPEEDDPTN, from the internal region of the protein sequence according to NP_000622.2; NP_038202.1.
Product Comments
This antibody is expected to recognise both reported isoforms (NP_000622.2 and NP_038202.1).
Peptide Sequence
C-KDFPEEDDPTN
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
4689
Product Grade
IHC Results
Paraffin embedded Human Tonsil and Spleen. Recommended concentration: 2.5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx. 38kDa band observed in lysates of cell line Daudi (calculated MW of 39.0kDa according to NP_000622.2 and 38.8kDa according to NP_038202.1). In transfected HEK293 transiently expressing P40PHOX a band of approx. 42kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.
Application Type
Pep-ELISA, WB, IHC

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