Goat Anti-B7-H4 Antibody

$464.00

Category: Primary Antibodies
SKU Unit Size Price

Select a unit size:

SKU: EB07942
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
V-set domain containing T cell activation inhibitor 1|immune costimulatory protein|T cell costimulatory molecule B7x|VCTN1|RP11-229A19.4|PRO1291|FLJ22418|B7h.5|B7X|B7S1|B7H4|B7-H4
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm and membranes/cell junctions of MCF7 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_078902.2; NP_001240778.1
Blocking Peptide
EBP07942
Immunogen
Peptide with sequence C-SKGKGNANLEYK, from the internal region of the protein sequence according to NP_078902.2; NP_001240778.1.
Peptide Sequence
C-SKGKGNANLEYK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog
Reactive Species
Human
Human Gene ID
79679
Mouse Gene ID
242122
Rat Gene ID
295322
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Western Blot
Approx 26+21kDa bands observed in HEK293 cell lysates, which were both successfully blocked by incubation with the immunizing peptide. An approx 26kDa and 21kDa band observed in Human Adrenal Gland lysates, and approx 27kDa in Human Pancreas lysates (calculated MW of 30.9kDa according to NP_078902.2 isoform 1 and 20.7kDa according to NP_001240778.1 isoform 2 ). Recommended concentration: 0.1-1µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, FC

Related products