Goat Anti-GCC185 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB07992
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
GRIP coiled-coil protein GCC185, (Ran-binding protein 2-like 4)|GRIP and coiled-coil domain-containing 2|CTCL tumor antigen se1-1|CLL-associated antigen KW-11|KIAA0336|GCC185|GRIP and coiled-coil domain containing 2|GCC2
Accession ID
NP_055450.1; NP_852118.1
Blocking Peptide
EBP07992
Immunogen
Peptide with sequence C-QKLTNKNNKIED, from the internal region of the protein sequence according to NP_055450.1; NP_852118.1.
Product Comments
This antibody is expected to recognise both reported isoforms (NP_055450.1and NP_852118.1).
Peptide Sequence
C-QKLTNKNNKIED
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Rat
Reactive Species
Human
Human Gene ID
9648
Product Grade
IHC Results
Paraffin embedded Human Spleen, Small Intestine and Kidney. Recommended concentration: 3.75µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Preliminary experiments gave an approx 60kDa band in Human Lymph Node and Testis lysates after 0.03µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 185kDa according to NP_055450.1 and of 196kDa according to NP_852118.1. The 60kDa band was successfully blocked by incubation with the immunizing peptide.
Application Type
Pep-ELISA, IHC

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