Goat Anti-GLuR5 / GRIK1 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB08000
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
GLUR5|GRIK1|glutamate receptor, ionotropic, kainate 1|EAA3|EEA3|GLR5|excitatory amino acid receptor 3
Accession ID
NP_000821.1; NP_783300.1
Blocking Peptide
EBP08000
Immunogen
Peptide with sequence QCKQTHPTNSTS, from the internal region (near the C Terminus) of the protein sequence according to NP_000821.1; NP_783300.1.
Product Comments
This antibody is expected to recognise both reported isoforms according to NP_000821.1 and NP_783300.1.
Peptide Sequence
QCKQTHPTNSTS
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat
Reactive Species
Human
Human Gene ID
2897
Mouse Gene ID
14805
Rat Gene ID
29559
Product Grade
IHC Results
Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:8000.
Western Blot
Approx 150kDa band observed in Human Brain (Cerebellum and Cerebral Cortex) lysates (calculated MW of 104kDa according to NP_000821.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Christensen et al , J Neurosci. 2004 Oct 13;24(41):8986-93.; PMID: 15483117). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour. An additional very strong band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide.
Application Type
Pep-ELISA, WB, IHC

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